The Crystallography Facility at Longwood Center
Mission:Our mission includes maintaining and providing training for crystallography equipment (liquid handlers, crystal hotel & imager, and X-Ray generator & detectors) for use by DFCI structural biology laboratories.
Equipment:Crystallization robots (Phoenix and NT8) Phoenix and NT8 liquid handlers are used in conjunction to deliver large and small volumes. Three-seat 96-well Intelli-plates and 384-well clear polystyrene plates (hanging drops) are provided, as well as common sparse-matrix source screens. Other crystallization screens might be available but will not necessarily be replenished.
Custom formulators (Formulator and NT8) Expansion or optimization plates are prepared using either the Formulator or the NT8. Common stock primary precipitant solutions and stock buffers are provided and maintained.
Automated plate microscopy/storage (Rock Imager 1000) Crystallization plates must be stored in the Rock Imager (in order to keep track of usage and associated costs). Users will be setup with their own, secure account in RockMaker software. When Imager plate capacity is reached, the oldest 20% of plates will automatically be moved into the adjoining room.
In-house X-Ray system (Microstar and Mar345) An in-house generate and detector system is available for rapid data-set collection or for rapid crystal evaluation. Training and certification is provided and required before use.
Access Fee:Effective 2017, an access fee will be implemented for the crystallography facility. The fee will allow access to the following instruments: Phoenix, NT8, RockImager, RockFormulator, microscope, Xray room equipment (generator and detectors), and a crystallography computer graphics station. The fee will also make available NT8 and Pheonix supplies (crystallization plates, sparse crystallization buffers, and sealers). But the following will not be provided: crystal harvesting pins, dewers, shipping containers, and associated equipment. The access fee will cover costs associated with service-contract-equivalence, supplies, and software licencing fees, regardless of frequency-of-use or whether users use their own or available supplies. Fee(2017): 4K$/year/lab. Contact us for inquiries.