Instruments and Equipment
Crystallization robots (Phoenix and NT8) Phoenix and NT8 liquid handlers are used in conjunction to deliver large and small volumes. Three-seat 96-well Intelli-plates and 384-well clear polystyrene plates (hanging drops) are provided, as well as common sparse-matrix source screens. Other crystallization screens might be available but will not necessarily be replenished.
Custom formulators (Formulator and NT8) Expansion or optimization plates are prepared using either the Formulator or the NT8. Common stock primary precipitant solutions and stock buffers are provided and maintained.
Automated plate microscopy/storage (Rock Imager 1000) Crystallization plates must be stored in the Rock Imager (in order to keep track of usage and associated costs). Users will be setup with their own, secure account in RockMaker software. When Imager plate capacity is reached, the oldest 20% of plates will automatically be moved into the adjoining room.
In-house X-Ray system (Microstar and Mar345) An in-house generate and detector system is available for rapid data-set collection or for rapid crystal evaluation. Training and certification is provided and required before use.
Bacterial and Insect/Baculoviral incubators Standard bacterial shaker/incubators with associated large-scale centrifuges. A dedicated baculovirus and insect-cell room includes fumehoods, incubators, microscope, centrifuges, and cell counters.
Protein purification systems (FPLCs) A pair of AKTA Pure 25L with inlet, column, and outlet valve kits are running specialized software to process high-throughput serial & tandem purification schemes.